Websmall-molecule sensors using naturally occurring highly stable three-way junction RNAs. We show that ligand-binding aptamers and fluorogenic aptamers can be inserted into … WebMar 10, 2024 · The authors describe a method for DNA target recognition and signal amplification that is based on the target-induced formation of a three way junction. The subsequent assembly of two DNA probes releases the inhibitory strand and triggers a downstream strand displacement amplification.
Cofactor-assisted three-way DNA junction-driven strand …
WebJan 1, 2024 · We, herein, describe a three-way junction (3WJ)-induced isothermal amplification (ThIsAmp) reaction for target nucleic acid detection. In this strategy, target nucleic acid induces the formation of 3WJ structure by associating a specially designed ThIsAmp template and ThIsAmp primer. Upon the format … WebNov 18, 2024 · Three-way junction-based metabolite sensors are constructed using multiple aptamers • The three-way junction sensor platform is a versatile approach for … client proofing sites for photographers
Naturally occurring three-way junctions can be repurposed as ...
WebJun 29, 2015 · ATP specifically binds with the split aptamers, bringing DNA1 and DNA2 close to each other, thus inducing the DNA three-way junction formation through the … WebMay 18, 2024 · Three-way junction-based metabolite sensors are constructed using multiple aptamers • The three-way junction sensor platform is a versatile approach for making RNA sensors • Three-way junction sensors detect SAM levels in live mammalian cells Summary Small molecules can be imaged in living cells using biosensors … WebOct 21, 2024 · The Duchenne muscular dystrophy (DMD) gene has a complex expression pattern regulated by multiple tissue-specific promoters and by alternative splicing (AS) of the resulting transcripts. Here, we used an RNAi-based approach coupled with DMD-targeted RNA-seq to identify RNA-binding proteins (RBPs) that regulate splicing of its skeletal … client protection fund scotland