Labchip charge variants
WebDec 7, 2015 · LabChip GXII HT Protein Charge Variant Kit Quick GuideNote: We highly recommend that first-time users read the full User Guide for this assay, before proceeding.Allow the Chip and Reagents to equilibrate to room temperature for 30 minutes before use.Protect the Dye Concentrate from exposure to light.Chip PreparationKeep the … WebApr 16, 2013 · High-Throughput Screening of mAb Charge Variants Using Microchip-CZE Authors: Tobias Wheeler, Lucy Sun, Rajendra Singh, Bahram Fathollahi and Hans Pirard Caliper - A PerkinElmer Company, Alameda, CA, USA Intermediate Assay Reproducibility Relative areas of basic, main, and acidic variants 1 Abstract Conventional vs. Microfluidic …
Labchip charge variants
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WebMay 1, 2014 · Wheeler et al. used a microchip zone electrophoresis device (LabChip GXII TM ) com- mercialized by Perkin Elmer to separate mAbs and their charge variants in less than 90 s, resulting in a 8 to 90 ... WebThe Protein Charge Variant Assay is for use with the LabChip GXII Touch instrument. LabChip GXII Touch instruments are for research use only and not for use in diagnostic …
WebHigh pI Charge Variant LabChips Table 2. Reagents Items at -20ºC Vial Quantity Labeling Buffer Orange 1 vial, 1 mL Dye Concentrate Blue 5 vials, 7 µL (24 samples/vial) Items at 2 - … WebATUM customer support scientists are available to discuss cloning strategies, gene design constraints, bioinformatics analyses, and other molecular biology/biotechnology concerns. Call Corporate Headquarters (Newark, California) +1 650 853 8347 Email We generally reply within a few hours. [email protected] Protein Services Assay Capabilities
WebPN CLS140162 Rev. C Protein Charge Variant Assay User Guide PerkinElmer, Inc. 6 Make sure the rims of the chip wells are clean and dry. 7 IMPORTANT: Ensure chip well 1 (waste well) is empty before placing the chip in the LabChip GXII Touch. Note: Each chip preparation is sufficient for running 96 samples. Preparing the Buffer Tube Buffer Web(Protein, Glycan, and Protein Charge Variant assays are only supported on LabChip GX II instruments.) 4 Type the operator's name in the Operator Name text box. 5 Select the name of the plate in the Plate Name drop-down list. To use a plate that is not listed in the Plate Name list, see “Adding a New Plate” on page 38.
WebThese differences allow for protein analysis and characterization by separation and identification. The LabChip ® GXII Touch ™ protein characterization system offers rapid peak quantification and quality control throughout the protein purification workflow. Analysis can be performed in as few as forty seconds per sample that deliver ... top rated blackwood laminate vinyl planksWebLabChip microfluidic-based assays consume just 150 nanoliters of sample per separation and are compatible with nucleic acid and protein samples in the low pg/μL and ng/mL concentration ranges, respectively. top rated bleach for bathroomWebZipChip’s charge variant assay alone gives you more information than other techniques. Same goes for other characterization assays. Whether you’re running intact charge variant analysis, reduced analysis, or peptide mapping, ZipChip works at every level Suggested Resources Protein Characterization Applications top rated blade putterWebApr 12, 2016 · Charge Variant Analysis: Using an HT protein charge variant kit from PerkinElmer (#CLS760670) on a LabChip GXII system (PerkinElmer), we analyzed charge … top rated bladeless fansWebscreening assay to analyze antibody charge variants on a microfluidic chip has been described.15 The separation in this method is achieved by zone electrophoresis, with separation times more than 10-fold faster than conventional, low-throughput methods. The assay is based on the commercially available microfluidic LabChip GXII platform (Caliper, top rated blanched laminate vinyl planksWebNov 9, 2024 · Charge variant (main peak level, acidic and basic isoforms) analysis was performed using imaged capillary isoelectric focusing (icIEF) technology with Maurice instrument (Protein Simple, USA). N-Glycans were released from Protein A purified antibody using PNGase F (Promega, USA). top rated blade sharpenerWebThe PerkinElmer LabChip charge variant analysis chips allow you to screen and analyze the charge variant profiles of protein samples fast and easily on LabChip GXII Touch™ HT … top rated blanched laminate hardwood floor