WebRecently, we developed TET-assisted Pyridine Borane Sequencing (TAPS), which is a mild, bisulfite-free method for base-resolution direct DNA methylation sequencing. Here we optimized TAPS for cfDNA (cfTAPS) to provide high-quality and high-depth whole-genome cell-free methylomes. We applied cfTAPS to 85 cfDNA samples from patients with ... WebDec 15, 2024 · locations in genomic DNA for many years[4]. More recently, TET-assisted pyridine borane sequencing (TAPS)[5] technology has overcome the problem of producing low-complexity sequences which is present in the destructive bisulfite treatment of BS-seq. Another NC technique that has gained traction is known as thiol (SH)-linked alkylation for …
TAPS: The Development of a Direct and Base-Resolution Sequencing …
WebJan 27, 2024 · Recently, we developed TET-assisted pyridine borane sequencing (TAPS) for bisulfite-free direct sequencing of 5mC and 5hmC. Here we demonstrate that two sister … WebTAPS could replace bisulphite sequencing as the new standard in DNA epigenetic analysis and could have wide applications in academic research and clinical diagnostics. Currently, we are applying TAPS to the following research areas: Epigenetic sequencing of the circulating cell-free DNA to develop a non-invasive early cancer detection assay; fritz 5530 fiber tiscali
Mapping the epigenetic modifications of DNA and RNA
WebJul 19, 2024 · Unlike other DNA methylation sequencing methods, TAPS does not rely on the harsh chemical bisulphite, which degrades a lot of the DNA sample. This allows TAPS to be used on much smaller amounts of DNA, such as those found in blood samples. In 2024, the Song lab adapted TAPS for targeted long-read sequencing. WebThe simple method of pyridine borane sequencing (PB-seq) can be used for 5fC/5caC detection. 115 Since unmodified C is converted into T base in the deamination-based technique, there are... WebMay 22, 2024 · The TET-assisted pyridine borane sequencing (TAPS) (Liu et al., 2024b) was introduced to detect 5mC and 5hmC, in which 5mC and 5hmC are firstly oxidized by TET to 5caC and then reduced to dihydrouracil (DHU) using pyridine borane with around 98% conversion efficiency, and subsequently readout as T after PCR amplifications (Fig. 1). It … fritz 6590 firmware