Tg gata1a:dsred

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http://movie.biologists.com/video/10.1242/dev.173757/video-1 WebDownload scientific diagram Effect of todralazine on recovery from chemically induced hemolytic anemia in Tg(gata1a: dsRed) zebrafish embryos. (a) Representative images …

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Web21 dic 2012 · Figure S1: Microinjection of miR-142a-3p morpholino (MO) in zebrafish embryos in Tg (fli1:EGFP, gata1a: dsRed) leads to intersegmental vessel (Se) remodeling. A - Graphical representation of dose dependent microinjection ranging from 10–500 µM of miR-142a-3p morpholino (MO) in 28–30 hpf Tg(fli1:EGFP, gata1a: dsRed) zebrafish … Web1 apr 2024 · In this study, Tg (drl: GFP) embryos at 24 hpf and Tg (gata1a: DsRed) embryos at 24/48/72 hpf were collected and fixed in 4% paraformaldehyde overnight, … bubbling air in oil

Notch signaling regulates venous arterialization during zebrafish …

Web23 apr 2024 · The decrease in gata1a expression was consistent with a decrease in the number of Gata1 + cells as demonstrated by the Tg(gata1a:dsRed) (Figure 5I, P = 2.7125e-07). Collectively, these data suggest that isoprenoids regulate RBC differentiation in a gata1a dependent manner. Download : Download high-res image (282KB) Web25 mag 2024 · Finally, the lymphatic versus blood vessel identity was functionally confirmed through time-lapse imaging of Tg (gata1a:dsRed) that showed erythrocyte flow only in … WebJournal of Vascular Anomalies (Mar 2024) . MAP2K1 Mutation in Zebrafish Endothelial Cells Causes Arteriovenous Shunts Preventable by MEK Inhibition bubbling acid

ZFIN Transgenic Construct: Tg3(gata1a:DsRed)

ZFIN Feature: sd2Tg - Zebrafish Information Network

Web1 giu 2016 · When gata1a + erythroblasts sorted from 28-hpf Tg(gata1a:DsRed) transgenic embryos were transplanted into 36-hpf wild-type recipient embryos (> 100 donor cells per recipient), the donor gata1a + cells could be detected by flow cytometry in recipients at 3 dpf but were no longer detected (n = 15) by 7–9 dpf, suggesting their incapability of survival … Web288 righe · Tg(gata1a:DsRed) ID ZDB-TGCONSTRCT-070117-38 Name Tg(gata1a:DsRed) Previous Names. Tg(gata1:dsRed) Tg(gata1a:dsRed) Type engineered_plasmid … bubbling and bloody witch’s brewWeb19 mag 2024 · To determine whether these mutants exhibit vascular defects, hif-1aa bns89 and hif-1ab bns90 were crossed into the Tg(kdrl:EGFP) s843;Tg(gata1a:DsRed) sd2 … express care clinics webpay

"Web1 mag 2014 · Pang et al. (2024) used the double transgenic Tg(kdrl: EGFP; gata1a: dsRed) to assess erythropoiesis and circulatory anomalies in embryos exposed to … " - Tg gata1a:dsred

Tg gata1a:dsred

Label-free imaging of red blood cells and oxygenation with color …

Web17 dic 2014 · GATA1, an erythroid-specific transcription factor, marks erythroid lineage 50 and regulates vital developmental processes in vertebrates, including hematopoiesis. 50 … WebEGFP/gata1a:dsRed) transgenic line, conﬁrmed the essential role of pank2 in the formation of the vascular sys-tem. The speciﬁcity of the morpholino-induced phenotype …

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WebTg (gata1a:DsRed) Type Transgenic insertion ( 1 ) Protocol embryos treated with DNA Lab of Origin The Zon Lab Current Source European Zebrafish Resource Center (EZRC) ( … WebTg (gata1:dsRed)sd2 ( 1) Affected Genomic Region. This feature is representative of one or more unknown insertion sites. Construct. Tg (gata1a:DsRed) Type. Transgenic insertion ( 1 ) Protocol. embryos treated with DNA.

Web28 lug 2024 · Quick scan of Tg(flk1:EGFP;gata1a:dsRed) embryos for calculating velocity of pruning branches at 48 hpf. (MP4) Browse Search pgen.1009690.s016.mp4(2.49 MB) … Web20 lug 2014 · Transgenic lines Tg(gata1a:DsRed) 22, Tg(kdrl:GFP) 23, Tg(myl7:GFP) 24, Tg(myl7:DsRed) 25 and Tg(UAS:NpHR-mCherry) 26 were used. Individual positive …

Web16 nov 2015 · Tg (fli1a:EGFP)y1; Tg (gata1a:dsRed)sd2 homozygous double transgenic zebrafish embryos at 22 hours post-fertilization (hpf) were pretreated with the targeted … Web25 mar 2015 · Tg(kdrl:EGFP); Tg(gata1a:dsRed) fish were treated with DAPT from 3.5 to 6.5 dpa. White arrows point to the distal end of the area of Tg(kdrl:EGFP) positive cells. The blood flow had already separated into three vessels at 6 dpa in both the control and some fin rays of the DAPT-treated fish.

Web9 mag 2014 · (C) Bright-field (DIC) and epifluorescent (GFP and DsRED) micrographs of Tg(kdrl:GFP) s843 /Tg(gata1:DsRED) sd2 embryos at 72 hpf that were injected with control, gata1a or gata2a morpholinos.

Web5 mar 2024 · Embryos of AB wild-type, Tg(hb9:GFP) , Tg(fli1:EGFP) , Tg(nflk:EGFP) , and Tg(gata1a:DsRed) lines were raised in egg water at 28.5°C with 0.003% 1-phenyl-2-thiourea to suppress pigmentation. After 72 h postfertilization (hpf), zebrafish are referred to as larvae as previously described . Zebrafish ... bubbling and frothing liquidsWebA Blood circulation in the intersegmental vessels of the control (A1–A3) and foxm1 MO injected (A4–A6) Tg(fli1a:GFP; gata1:dsRed) zebrafish larvae at 72 hpf. “1, 2, 3,” … express care crescent cityWeb20 feb 2024 · Cells from TgBAC(tcf21:H2B-Dendra2) ox182 were sorted in four separate FACS sessions, GFP positive and DsRed positive cells from Tg(kdrl:GFP; gata1a:DsRed) and GFP positive cells from Tg(myl7:eGFP) f1 were sorted in one session, cells in all other conditions were sorted separately in one session each. express care crooked oakWeb11 feb 2024 · We reconstructed red blood cells (RBCs) (labeled with Tg (gata1a:dsRed)) and beating cardiomyocytes (nuclei were labeled with Tg (myl7:nls-gfp)) in four … express care college park mdWeb7 dic 2024 · (E,F) One-cell stage zebrafish eggs of Tg(gata1a:dsRED) were microinjected with control or full-length S RNAs and hemorrhages were analyzed at 48 hpf by fluorescence microscopy. The data are shown as the percentage of larvae showing hemorrhages. Representative images of the different experimental groups showing the hemorrhages ... bubbling air freshenerWebgata1a编码的GATA结合蛋白1a（GATAbinding protein 1a）具有序列特异性双链DNA结合及转录活性，参与造血、基因表达调控、髓样细胞分化等过程。在人类中，该基因的同源 … express care crescent city fl 32112Web11 ott 2013 · We performed time-lapse analysis of the eye vasculature between 32 hours post fertilization (hpf) and 48 hpf in Tg(kdrl:Hsa.HsRAS-mcherry) sa916; Tg(fli1a: ... Still images of time-lapse of Tg(kdrl:EGFP) s843; Tg(gata1a:DsRed) sd2 from 34–48 hpf. White brackets indicate dorsal CrDI, purple arrow indicates NCA. bubbling away crossword