Ufd1 antibody
Web7 Apr 2024 · Ubiquitin Fusion-Degradation Protein-1 Like (Ufd1l) encoded by the gene UFD1 serves as a ubiquitin recognition factor linking the ubiquitination and degradation process … WebThe UFD1 protein was originally characterized in the yeast S. cerevisiae and subsequently, the human homolog UFD1 or UFD1L was identified. In vitro, UFD1 attenuates the degradation of Ub-fusions, which have a proline or valine residue substituted at the Gly76 moiety, by the selective multiubiquitination of the Ub chain of the Ub-conjugate.
Ufd1 antibody
Did you know?
Web10615-1-AP targets UFD1L in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. … WebThe UFD1L Antibody from LifeSpan BioSciences is a Rabbit Polyclonal antibody to UFD1. This antibody recognizes Human antigen. The UFD1L Antibody has been validated for the …
Web29 Sep 2016 · Reagents and antibodies. DMEM, cycloheximide, anti-FLAG M2 affinity gels, and mouse monoclonal antibodies (mAbs) to α-tubulin and FLAG were purchased from Sigma-Aldrich. ... Ufd1 is colored in pink, Npl4 in blue, and p97 in gray. For simplicity, the binding of SHP (Ufd1) and UBD (Npl4) are pictured together on a single p97 N domain. … WebImmunoblotting of the bound fraction and input (cell lysate) with antibodies against GS or ubiquitin revealed that inhibition of either p97 or the proteasome in glutamine-treated cells resulted in a significant increase in ubiquitin-conjugated GS forms, which were deconjugated with the deubiquitylating enzyme ubiquitin C-terminal hydrolase 2 …
WebRabbit polyclonal antibody anti-UFD1 is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications. Web1 May 2000 · Anti-Ufd1 recognizes a single band at 42 kDa. Both recombinant proteins are slightly bigger than the endogenous proteins because of the His-tag and the His-Ufd1 fraction contains proteolytic degradation products.
Web11 Apr 2024 · The membranes were incubated with the primary antibodies prepared in TBS-Tween containing 5% BSA for 1-2 h at room temperature or 14–16 h at 4°C. Subsequently, …
Web9 Jan 2007 · ( Left Bottom) Ufd1-Npl4 was incubated with monoclonal IgGs directed against the N-terminal UT3 fragment of Ufd1. The antibody (indicated with a bracket) is binding … swiss info impressumWeb13 Nov 2007 · Function. Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1, … swiss in canadaWebMouse monoclonal antibody to UFD1. Datasheet; Select Product Size swiss influencerWeb10 Oct 2010 · The anti-Ufd1 siRNA were purchased from Ambion (Austin, TX). The targeting sequence is 5′-CCAACUCAGCCGACUUAAC. Bovine ubiquitin was purchased from Sigma. MG132 was purchased from Calbiochem. DeoxyBigCHAP was purchase from Dojindo (Rockville, MD). MHC HC and p97 antibodies were described previously ( 24 ). swiss infoliniaWeb17 Jul 2010 · Coprecipitated endogenous Npl4, Ufd1, p47 and ubiquitinated proteins were examined by Western blot analysis. Npl4 and Ufd1 were coprecipitated more potently with all tested IBMPFD‐VCPs than with wt‐VCP ( Fig. 5A ). swissinfo front pageWeb20 Sep 2024 · Here, we found that the serine 229 residue located in the Ufd1 SHP box is phosphorylated in vitro and in vivo by cyclic adenosine monophosphate-dependent protein kinase A (PKA), with this process being enhanced by either forskolin (an adenylyl cyclase activator) or calyculin A (a protein phosphatase inhibitor). Moreover, a phosphomimetic … swiss in californiaWebExpression of UFD1 in cancer - Summary - The Human Protein Atlas DISEASE UFD1 PATHOLOGY CANCER ANTIBODIES AND VALIDATION Dictionary Human pathology PROGNOSTIC SUMMARYi Prognostic marker in renal … swissinfo.ch 日本語